Synchrotron SAXS data from solutions of TRIM25/pre-let-7 in 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH 6.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample was injected onto a GE Superdex 200 Increase 10/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection concentration: UNKNOWN. Sample injection volume: UNKNOWN. SEC column flow rate: UNKNOWN. Total X-ray exposure time and number of individual SEC-SAXS data frames used for processing: UNKNOWN.
s, nm-1
