E3 ubiquitin/ISG15 ligase TRIM25

Kevin Haubrich.

SASDK98 – E3 ubiquitin/ISG15 ligase TRIM25 bound to pre-let-7-a-1@1 RNA (TRIM25/pre-let-7): SEC-SAXS

E3 ubiquitin/ISG15 ligase TRIM25
MWexperimental 109 kDa
MWexpected 109 kDa
log I(s) 2.16×101 2.16×100 2.16×10-1 2.16×10-2
E3 ubiquitin/ISG15 ligase TRIM25 pre-let-7-a-1@1 small angle scattering data  s, nm-1
ln I(s)
E3 ubiquitin/ISG15 ligase TRIM25 pre-let-7-a-1@1 Guinier plot ln 2.17×101 Rg: 5.7 nm 0 (5.7 nm)-2 s2
E3 ubiquitin/ISG15 ligase TRIM25 pre-let-7-a-1@1 Kratky plot 1.104 0 3 sRg
Dmax: 23 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of TRIM25/pre-let-7 in 20 mM MES, 75 mM NaCl, 1 mM TCEP, pH 6.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample was injected onto a GE Superdex 200 Increase 10/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample injection concentration: UNKNOWN. Sample injection volume: UNKNOWN. SEC column flow rate: UNKNOWN. Total X-ray exposure time and number of individual SEC-SAXS data frames used for processing: UNKNOWN.

E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   50.1 kDa
UniProt   Q14258 (189-630)
Sequence   FASTA
pre-let-7-a-1@1 (pre-let-7)
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   8.9 kDa
Sequence   FASTA