The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences

Lixin Fan.

SASDKD8 – LuxR family transcriptional regulator, SmcR

LuxR family transcriptional regulator
MWexperimental 46 kDa
MWexpected 52 kDa
VPorod 67 nm3
log I(s) 1.29×10-1 1.29×10-2 1.29×10-3 1.29×10-4
LuxR family transcriptional regulator small angle scattering data  s, nm-1
ln I(s)
LuxR family transcriptional regulator Guinier plot ln 1.30×10-1 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
LuxR family transcriptional regulator Kratky plot 1.104 0 3 sRg
p(r)
LuxR family transcriptional regulator pair distance distribution function Rg: 3.2 nm 0 Dmax: 12.0 nm

Data validation


Fits and models


log I(s)
 s, nm-1
LuxR family transcriptional regulator CHIMERA model
LuxR family transcriptional regulator CHIMERA model

Synchrotron SAXS data from solutions of the LuxR family transcriptional regulator, SmcR, in 200 mM NaCl, 25 mM Tris, pH 7.5 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.09322 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 45 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

LuxR family transcriptional regulator (SmcR)
Mol. type   Protein
Organism   Vibrio vulnificus
Olig. state   Other
Mon. MW   25.9 kDa
 
UniProt   Q9L8G8 (1-205)
Sequence   FASTA