The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences

Lixin Fan Submitted to SASBDB: 2021 Feb 15
Published in SASBDB:

SASDKD8 – LuxR family transcriptional regulator, SmcR

LuxR family transcriptional regulator experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator, 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, pH: 7.5
Experiment: SAXS data collected at 12ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 8
RgGuinier 3.1 nm
Dmax 12.0 nm
VolumePorod 67 nm3

SASDKE8 – LuxR family transcriptional regulator, SmcR N55I mutant

LuxR family transcriptional regulator, N55I experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator, N55I, 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 11
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 45 nm3