The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences.

Newman JD, Russell MM, Fan L, Wang YX, Gonzalez-Gutierrez G, van Kessel JC
Nucleic Acids Res 49(10):5967-5984 (2021 Jun 4)
PMID: 34023896
doi: 10.1093/nar/gkab387 		Submitted to SASBDB: 2021 Feb 15
Published in SASBDB:

SASDKD8 – LuxR family transcriptional regulator, SmcR

LuxR family transcriptional regulator experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator, 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 8
RgGuinier 3.1 nm
Dmax 12.0 nm
VolumePorod 67 nm3

SASDKE8 – LuxR family transcriptional regulator, SmcR N55I mutant

LuxR family transcriptional regulator, N55I experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator, N55I, 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 11
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 45 nm3