The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences

Lixin Fan.

SASDKE8 – LuxR family transcriptional regulator, SmcR N55I mutant

LuxR family transcriptional regulator, N55I
MWexperimental 42 kDa
MWexpected 52 kDa
VPorod 45 nm3
log I(s) 2.72×10-2 2.72×10-3 2.72×10-4 2.72×10-5
LuxR family transcriptional regulator, N55I small angle scattering data  s, nm-1
ln I(s)
LuxR family transcriptional regulator, N55I Guinier plot ln 2.72×10-2 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
LuxR family transcriptional regulator, N55I Kratky plot 1.104 0 3 sRg
p(r)
LuxR family transcriptional regulator, N55I pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.1 nm

Data validation


Fits and models


log I(s)
 s, nm-1
LuxR family transcriptional regulator, N55I CHIMERA model
LuxR family transcriptional regulator, N55I CHIMERA model

Synchrotron SAXS data from solutions of the LuxR family transcriptional regulator, SmcR N55I mutant, in 200 mM NaCl, 25 mM Tris, 1 mM DTT, 0.5 mM EDTA, pH 7.5 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.09322 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 45 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

LuxR family transcriptional regulator, N55I (SmcR N55I)
Mol. type   Protein
Organism   Vibrio vulnificus
Olig. state   Other
Mon. MW   25.9 kDa
 
UniProt   Q9L8G8 (1-205)
Sequence   FASTA