The solution structures of higher-order human telomere G-quadruplex multimers.

Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO, Nucleic Acids Res (2021) Europe PMC

SASDKG3 – Human wild-type telomere 48-mer d(TTAGGG)8

Human Telomere Repeat (TTAGGG)8
MWexperimental 16 kDa
MWexpected 15 kDa
VPorod 16 nm3
log I(s) 3.17×10-2 3.17×10-3 3.17×10-4 3.17×10-5
Human Telomere Repeat (TTAGGG)8 small angle scattering data  s, nm-1
ln I(s)
Human Telomere Repeat (TTAGGG)8 Guinier plot ln 3.18×10-2 Rg: 1.9 nm 0 (1.9 nm)-2 s2
Human Telomere Repeat (TTAGGG)8 Kratky plot 1.104 0 3 sRg
Human Telomere Repeat (TTAGGG)8 pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Telomere Repeat (TTAGGG)8 DAMMIN model

Synchrotron SAXS data from solutions of Human wild-type telomere 48-mer d(TTAGGG)8 in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 13 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. Four successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human Telomere Repeat (TTAGGG)8 (Tel48)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   15.3 kDa
Sequence   FASTA