The solution structures of higher-order human telomere G-quadruplex multimers.

Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO, Nucleic Acids Res (2021) Europe PMC

SASDKH3 – Human wild-type telomere 72mer d(TTAGGG)12

Human Telomere Repeat (TTAGGG)12
MWexperimental 24 kDa
MWexpected 23 kDa
VPorod 26 nm3
log I(s) 3.74×10-3 3.74×10-4 3.74×10-5 3.74×10-6
Human Telomere Repeat (TTAGGG)12 small angle scattering data  s, nm-1
ln I(s)
Human Telomere Repeat (TTAGGG)12 Guinier plot ln 3.74×10-3 Rg: 2.5 nm 0 (2.5 nm)-2 s2
Human Telomere Repeat (TTAGGG)12 Kratky plot 1.104 0 3 sRg
Human Telomere Repeat (TTAGGG)12 pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Telomere Repeat (TTAGGG)12 DAMMIF model

Synchrotron SAXS data from solutions of Human wild-type telomere 72mer d(TTAGGG)12 in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 10 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. Four successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human Telomere Repeat (TTAGGG)12 (Tel72)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   22.9 kDa
Sequence   FASTA