| MWexperimental | 83 | kDa |
| MWexpected | 122 | kDa |
| VPorod | 245 | nm3 |
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log I(s)
3.99×103
3.99×102
3.99×101
3.99×100
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s, nm-1
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Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.
Kasiliauskaite A, Kubicek K, Klumpler T Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R, Nucleic Acids Res (2022) Europe PMCSASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant
Transcription elongation factor SPT6 - ΔtSH2 variant|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
Transcription elongation factor SPT6 - ΔtSH2 variant
in
25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 1.24 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 15°C.
20 successive
0.045 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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