Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.

Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R, Nucleic Acids Res (2022) Europe PMC

SASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant

Transcription elongation factor SPT6 - ΔtSH2 variant
MWexperimental 83 kDa
MWexpected 122 kDa
VPorod 245 nm3
log I(s) 3.99×103 3.99×102 3.99×101 3.99×100
Transcription elongation factor SPT6 - ΔtSH2 variant small angle scattering data  s, nm-1
ln I(s)
Transcription elongation factor SPT6 - ΔtSH2 variant Guinier plot ln 4.00×103 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription elongation factor SPT6 - ΔtSH2 variant Kratky plot 1.104 0 3 sRg
p(r)
Transcription elongation factor SPT6 - ΔtSH2 variant pair distance distribution function Rg: 4.2 nm 0 Dmax: 13.9 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔtSH2 variant Rg histogram Rg, nm
Transcription elongation factor SPT6 - ΔtSH2 variant EOM/RANCH model
Transcription elongation factor SPT6 - ΔtSH2 variant EOM/RANCH model
Transcription elongation factor SPT6 - ΔtSH2 variant EOM/RANCH model
Transcription elongation factor SPT6 - ΔtSH2 variant EOM/RANCH model

log I(s)
 s, nm-1
Transcription elongation factor SPT6 - ΔtSH2 variant CORAL model

Synchrotron SAXS data from solutions of Transcription elongation factor SPT6 - ΔtSH2 variant in 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 1.24 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 15°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcription elongation factor SPT6 - ΔtSH2 variant (SPT6 ΔtSH2)
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   122.2 kDa
 
UniProt   P23615
Sequence   FASTA
 
PDB ID   3PSI
 
PDB ID   3PSI
 
PDB ID   3PSI
 
PDB ID   3PSI
 
PDB ID   3PSI