Synchrotron SAXS
data from solutions of
Autophagy-related protein 23
in
20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH 7.4
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a wavelength of λ = 1.127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 20.00 μl sample
at 6 mg/ml was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
10 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
