Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer.

Gupta K Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG, Nucleic Acids Res 49(13):7644-7664 (2021) Europe PMC

SASDL45 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (6 mg/mL)

Survival motor neuron-like protein 1
Survival of motor neuron protein-interacting protein yip11
Gcn4p
MWexperimental 115 kDa
MWexpected 89 kDa
VPorod 325 nm3
log I(s) 2.07×102 2.07×101 2.07×100 2.07×10-1
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p small angle scattering data  s, nm-1
ln I(s)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Guinier plot ln 2.07×102 Rg: 6.6 nm 0 (6.6 nm)-2 s2
(sRg)2I(s)/I(0)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot 1.104 0 √3 sRg
p(r)
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p pair distance distribution function Rg: 6.8 nm 0 Dmax: 22.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of the survival motor neuron SMN(GCN4IL) Chimera•Gemin2 complex in 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, were collected on the X21 beam line at the NSLS (Upton, USA) using a MAR165 detector at a sample-detector distance of 0.8 m and at a wavelength of λ = 0.1239 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 4°C. Three successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Survival motor neuron-like protein 1 (spSMN)
Mol. type   Protein
Organism   Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Olig. state   Dimer
Mon. MW   13.2 kDa
 
UniProt   Q09808 (1-117)
Sequence   FASTA
 
Survival of motor neuron protein-interacting protein yip11 (spGemin2)
Mol. type   Protein
Organism   Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Olig. state   Dimer
Mon. MW   27.0 kDa
 
UniProt   P0CU08 (1-235)
Sequence   FASTA
 
Gcn4p (GCN4(IL))
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain JAY291)
Olig. state   Dimer
Mon. MW   4.1 kDa
Sequence   FASTA