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42 hits found for Gupta

SASDEB2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73E

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 19.8 nm
Dmax 23.4 nm

SASDEC2 – Cell wall synthesis protein Wag31: Polymer tbWag31 T73A

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 15
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 21.8 nm
Dmax 13.6 nm

SASDFF2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.5 nm

SASDFG2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 11.4 nm

SASDFH2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.5 nm

SASDFJ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.1 nm

SASDFK2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 9.9 nm

SASDFL2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 9.0 nm

SASDFM2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 9.8 nm

SASDFN2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 9.2 nm

SASDFP2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 8.1 nm

SASDFQ2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 8.1 nm

SASDFR2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 15.2 nm

SASDFS2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 280-284)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 13.2 nm

SASDFT2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 285-289)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 15.3 nm

SASDJT2 – DNA-directed RNA polymerase (RPO41)

DNA-directed RNA polymerase experimental SAS data
DNA-directed RNA polymerase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.4 nm
Dmax 12.5 nm
VolumePorod 295 nm3

SASDFU2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 290-294)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 14.5 nm

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1)

DNA-directed RNA polymeraserRNA adenine N (6) methyl transferase experimental SAS data
DNA-directed RNA polymerase rRNA adenine N (6) methyl transferase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
rRNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 5.5 nm
Dmax 15.0 nm
VolumePorod 393 nm3

SASDFV2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 295-299)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 12.1 nm

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1)

DNA-directed RNA polymeraseMitochondrial transcription factor 1 experimental SAS data
DNA-directed RNA polymerase Mitochondrial transcription factor 1 Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7
PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.5 nm
Dmax 14.4 nm
VolumePorod 292 nm3

SASDFW2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 300-304)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 13.1 nm

SASDFX2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 11.1 nm

SASDFY2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 310-314)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.6 nm

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 , 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
...Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.2 nm

SASDL35 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (12 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, NSLS on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 23.3 nm
VolumePorod 320 nm3

SASDL45 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (6 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, NSLS on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 22.9 nm
VolumePorod 325 nm3

SASDL55 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (4 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, NSLS on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.4 nm
Dmax 21.9 nm
VolumePorod 315 nm3

SASDB65 – Tyrosine-protein phosphatase (YopH)/putative yopH targeting protein (SycH) heterotrimeric complex

SycH putative yopH targeting proteinTyrosine-protein phosphatase YopH experimental SAS data
CRYSOL model
Sample: SycH putative yopH targeting protein dimer, 32 kDa Yersinia pseudotuberculosis protein
Tyrosine-protein phosphatase YopH monomer, 14 kDa Yersinia pseudotuberculosis protein
Buffer: 50 mM HEPES 2mM TCEP, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Aug 1
Global Disordering in Stereo-Specific Protein Association Biophysical Journal 112(3):33a (2017)
Gupta A, Reinartz I, Spilotros A, Jonna V, Hofer A, Svergun D, Schug A, Wolf-Watz M
RgGuinier 3.0 nm
Dmax 12.5 nm
VolumePorod 89 nm3

SASDL65 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (3 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, NSLS on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.3 nm
Dmax 19.9 nm
VolumePorod 315 nm3

SASDL75 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Tetramer analyzed by SVD-EFA SEC-SAXS

Survival motor neuron proteinMaltose/maltodextrin-binding periplasmic protein experimental SAS data
Survival motor neuron protein Maltose/maltodextrin-binding periplasmic protein Kratky plot
Sample: Survival motor neuron protein tetramer, 15 kDa Drosophila melanogaster protein
Maltose/maltodextrin-binding periplasmic protein tetramer, 174 kDa Escherichia coli (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 3.9 nm
Dmax 11.3 nm
VolumePorod 340 nm3

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic proteinSurvival motor neuron protein experimental SAS data
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot
Sample: Maltose/maltodextrin-binding periplasmic protein octamer, 347 kDa Escherichia coli (strain … protein
Survival motor neuron protein octamer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 5.6 nm
Dmax 17.9 nm
VolumePorod 800 nm3

SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS

Protein Gemin2Survival motor neuron protein experimental SAS data
Protein Gemin2 Survival motor neuron protein Kratky plot
Sample: Protein Gemin2 octamer, 230 kDa Drosophila melanogaster protein
Survival motor neuron protein octamer, 197 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 7.8 nm
Dmax 28.7 nm
VolumePorod 2660 nm3

SASDHC6 – C-terminal domain-like carotenoid protein (CCP2), apo-dimer

C-terminal domain-like carotenoid protein experimental SAS data
BILBOMD model
Sample: C-terminal domain-like carotenoid protein dimer, 33 kDa Tolypothrix sp. PCC … protein
Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15
Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
...Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C
RgGuinier 2.5 nm
Dmax 9.1 nm
VolumePorod 55 nm3

SASDHD6 – C-terminal domain-like carotenoid protein (CCP2), holo-dimer

C-terminal domain-like carotenoid protein experimental SAS data
BILBOMD model
Sample: C-terminal domain-like carotenoid protein dimer, 36 kDa Tolypothrix sp. PCC … protein
Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15
Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
...Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C
RgGuinier 2.5 nm
Dmax 7.9 nm
VolumePorod 62 nm3

SASDHE6 – C-terminal domain-like carotenoid protein (CCP2), apo-tetramer

C-terminal domain-like carotenoid protein experimental SAS data
BILBOMD model
Sample: C-terminal domain-like carotenoid protein tetramer, 66 kDa Tolypothrix sp. PCC … protein
Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15
Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
...Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C
RgGuinier 3.2 nm
Dmax 10.0 nm
VolumePorod 110 nm3

SASDHF6 – C-terminal domain-like carotenoid protein (CCP2), holo-tetramer

C-terminal domain-like carotenoid protein experimental SAS data
BILBOMD model
Sample: C-terminal domain-like carotenoid protein tetramer, 73 kDa Tolypothrix sp. PCC … protein
Buffer: 10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15
Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
...Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C
RgGuinier 3.3 nm
Dmax 12.1 nm
VolumePorod 157 nm3

SASDDU8 – Multidomain architecture of the estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains

Estrogen receptorERE1ERE2EstradiolhERa peptide1hERa peptide2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor dimer, 85 kDa protein
ERE1 monomer, 6 kDa Homo sapiens DNA
ERE2 monomer, 6 kDa Homo sapiens DNA
Estradiol dimer, 0 kDa
hERa peptide1 monomer, 2 kDa protein
hERa peptide2 monomer, 2 kDa protein
Buffer: 10 mM CHES (pH9.5), 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol, pH: 9.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Aug 10
Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains. Nat Commun 9(1):3520 (2018)
...Gupta S, Ralston C, Kao HY, Chance MR, Yang S
RgGuinier 3.8 nm
Dmax 11.5 nm

SASDCT2 – Bromodomain-containing protein 2 (BRD2) tandem bromodomains

Bromodomain-containing protein 2 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.7 nm
Dmax 21.0 nm
VolumePorod 220 nm3

SASDCS2 – Bromodomain-containing protein 3 (BRD3) tandem bromodomains

Bromodomain-containing protein 3 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.2 nm
Dmax 21.5 nm
VolumePorod 210 nm3

SASDCR2 – Bromodomain-containing protein 4 (BRD4) tandem bromodomains

Bromodomain-containing protein 4 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 4 monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 251 nm3

SASDCU2 – Bromodomain testis-specific protein (BRDT) tandem bromodomains

Bromodomain testis-specific protein experimental SAS data
DAMMIN model
Sample: Bromodomain testis-specific protein monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 200 nm3