Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy.

Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ, Cell Rep 39(3):110702 (2022) Europe PMC

SASDL49 – Autophagy-related protein 23 L171A,I182A,L189A mutant

Autophagy-related protein 23 LIL Mutant
MWexperimental 51 kDa
MWexpected 52 kDa
VPorod 98 nm3
log I(s) 4.31×101 4.31×100 4.31×10-1 4.31×10-2
Autophagy-related protein 23 LIL Mutant small angle scattering data  s, nm-1
ln I(s)
Autophagy-related protein 23 LIL Mutant Guinier plot ln 4.32×101 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Autophagy-related protein 23 LIL Mutant Kratky plot 1.104 0 √3 sRg
p(r)
Autophagy-related protein 23 LIL Mutant pair distance distribution function Rg: 4.8 nm 0 Dmax: 17.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Autophagy-related protein 23 L171A,I182A,L189A mutant in 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a wavelength of λ = 1.127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 20.00 μl sample at 6 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 20°C. Six successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Autophagy-related protein 23 LIL Mutant
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   51.9 kDa
 
UniProt   Q06671 (1-453)
Sequence   FASTA