Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer.

Gupta K Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG, Nucleic Acids Res 49(13):7644-7664 (2021) Europe PMC

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic protein
Survival motor neuron protein
MWexperimental 345 kDa
MWexpected 378 kDa
VPorod 800 nm3
log I(s) 7.41×103 7.41×102 7.41×101 7.41×100
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein small angle scattering data  s, nm-1
ln I(s)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Guinier plot ln 7.41×103 Rg: 5.6 nm 0 (5.6 nm)-2 s2
(sRg)2I(s)/I(0)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot 1.104 0 √3 sRg
p(r)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein pair distance distribution function Rg: 5.3 nm 0 Dmax: 17.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of an MBP-Survival motor neuron protein fusion in 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Component SAXS profile calculated using singular value decomposition evolving factor analysis (SVD-EFA).

Maltose/maltodextrin-binding periplasmic protein (MBP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   43.4 kDa
 
UniProt   P0AEX9 (1-396)
Sequence   FASTA
 
Survival motor neuron protein (SMN)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Octamer
Mon. MW   3.8 kDa
 
UniProt   Q9VV74 (186-220)
Sequence   FASTA