Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer.

Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG, Nucleic Acids Res 49(13):7644-7664 (2021) Europe PMC

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic protein
Survival motor neuron protein
MWexperimental 345 kDa
MWexpected 378 kDa
VPorod 800 nm3
log I(s) 7.41×103 7.41×102 7.41×101 7.41×100
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein small angle scattering data  s, nm-1
ln I(s)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Guinier plot ln 7.41×103 Rg: 5.6 nm 0 (5.6 nm)-2 s2
(sRg)2I(s)/I(0)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot 1.104 0 √3 sRg
p(r)
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein pair distance distribution function Rg: 5.3 nm 0 Dmax: 17.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of an MBP-Survival motor neuron protein fusion in 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Component SAXS profile calculated using singular value decomposition evolving factor analysis (SVD-EFA).

Maltose/maltodextrin-binding periplasmic protein (MBP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   43.4 kDa
 
UniProt   P0AEX9 (1-396)
Sequence   FASTA
 
Survival motor neuron protein (SMN)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Octamer
Mon. MW   3.8 kDa
 
UniProt   Q9VV74 (186-220)
Sequence   FASTA