Gupta K
Wen Y,
Ninan NS,
Raimer AC,
Sharp R,
Spring AM,
Sarachan KL,
Johnson MC,
Van Duyne GD,
Matera AG,
Nucleic Acids Res
49(13):7644-7664
(2021)
Europe PMC
SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS
Synchrotron SAXS data from solutions of an MBP-Survival motor neuron protein fusion in 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 2.5 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 4°C. 589 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Component SAXS profile calculated using singular value decomposition evolving factor analysis (SVD-EFA).
s, nm-1
