| MWI(0) | 65 | kDa |
| MWexpected | 75 | kDa |
| VPorod | 132 | nm3 |
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log I(s)
1.54×101
1.54×100
1.54×10-1
1.54×10-2
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s, nm-1
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Conformational Changes of RORĪ³ During Response Element Recognition and Coregulator Engagement
Strutzenberg T, Zhu Y, Novick S, Garcia-Ordonez R, Doebelin C, He Y, Ra Chang M, Kamenecka T, Edwards D, Griffin P, Journal of Molecular Biology :167258 (2021) DOISASDLE5 – RORg2 bound to a Classic-RORgamma Response Element
Retinoid-related orphan receptor-gammaClassic-RORgamma Response Element
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
RORg2 bound to a Classic-RORgamma Response Element
in
25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.14 mg/ml was measured
at 10°C.
33 successive
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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