Conformational Changes of RORĪ³ During Response Element Recognition and Coregulator Engagement

Strutzenberg T, Zhu Y, Novick S, Garcia-Ordonez R, Doebelin C, He Y, Ra Chang M, Kamenecka T, Edwards D, Griffin P
Journal of Molecular Biology :167258 (2021 Sep)

doi: 10.1016/j.jmb.2021.167258 		Submitted to SASBDB: 2021 May 25
Published in SASBDB:

SASDLE5 – RORg2 bound to a Classic-RORgamma Response Element

Retinoid-related orphan receptor-gammaClassic-RORgamma Response Element experimental SAS data
RORg2 bound to a Classic-RORgamma Response Element Rg histogram
Sample: Retinoid-related orphan receptor-gamma monomer, 56 kDa Homo sapiens protein
Classic-RORgamma Response Element dimer, 19 kDa Homo sapiens DNA
Buffer: 25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 20
RgGuinier 5.5 nm
Dmax 22.9 nm
VolumePorod 132 nm3

SASDLF5 – RORg2 bound to a Variant-RORgamma Response Element

Retinoid-related orphan receptor-gammaVariant-RORgamma Response Element experimental SAS data
RORg2 bound to a Variant-RORgamma Response Element Rg histogram
Sample: Retinoid-related orphan receptor-gamma monomer, 56 kDa Homo sapiens protein
Variant-RORgamma Response Element dimer, 18 kDa Homo sapiens DNA
Buffer: 25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 20
RgGuinier 4.4 nm
Dmax 22.1 nm
VolumePorod 112 nm3