The suppressor of copper sensitivity protein C from Caulobacter crescentus is a trimeric disulfide isomerase that binds copper(I) with subpicomolar affinity

Petit G, Hong Y, Djoko K, Whitten A, Furlong E, McCoy A, Gulbis J, Totsika M, Martin J, Halili M, Acta Crystallographica Section D Structural Biology 78(3):337-352 (2022) DOI

SASDLE9 – Suppressor of Copper Sensitivity C protein from Caulobacter crescentus

Thioredoxin domain-containing protein
MWexperimental 79 kDa
MWexpected 73 kDa
VPorod 97 nm3
log I(s) 4.51×10-2 4.51×10-3 4.51×10-4 4.51×10-5
Thioredoxin domain-containing protein small angle scattering data  s, nm-1
ln I(s)
Thioredoxin domain-containing protein Guinier plot ln 4.52×10-2 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Thioredoxin domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
Thioredoxin domain-containing protein pair distance distribution function Rg: 3.9 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Suppressor of Copper Sensitivity C protein from Caulobacter crescentus Rg histogram Rg, nm
Thioredoxin domain-containing protein EOM/RANCH model
Thioredoxin domain-containing protein EOM/RANCH model
Thioredoxin domain-containing protein EOM/RANCH model
Thioredoxin domain-containing protein EOM/RANCH model
log I(s)
 s, nm-1
Thioredoxin domain-containing protein CORAL model
X-ray synchrotron radiation scattering data from solutions of Suppressor of Copper Sensitivity C protein (ScsC) from Caulobacter crescentus in 25 mM HEPES 150mM NaCl, pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Dectris/Pilatus3 S 2M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) with a Superdex 200 5/150 GL column and sheath flow was used. Approximately 60 uL of protein at 5.6 mg/mL was injected onto the column. Twenty eight successive 1 second frames were averaged across the elution peak, where the radius of gyration appeared stable, and the average concentration across this range is estimated to be approximately 1.7 mg/mL (not measured). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from rigid-body modelling using CORAL (top) and a representative ensemble of four trimeric ScsC structures determined using ensemble optimization method (EOM). The Rg and Dmax distributions derived from EOM are included in the full entry zip archive.

Thioredoxin domain-containing protein (CcScsC)
Mol. type   Protein
Organism   Caulobacter vibrioides (strain ATCC 19089 / CB15)
Olig. state   Trimer
Mon. MW   24.4 kDa
 
UniProt   Q9A747 (23-246)
Sequence   FASTA
 
PDB ID   7RGV