TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life.

Davé E, Durrant O, Dhami N, Compson J, Broadbridge J, Archer S, Maroof A, Whale K, Menochet K, Bonnaillie P, Barry E, Wild G, Peerboom C, Bhatta P, Ellis M, Hinchliffe M, Humphreys DP, Heywood SP, MAbs 15(1):2160229 (2023) Europe PMC

SASDM45 – TrYbe® trispecific antibody

Tri-specific antibody
MWI(0) 100 kDa
MWexpected 102 kDa
VPorod 157 nm3
log I(s) 1.20×10-1 1.20×10-2 1.20×10-3 1.20×10-4
Tri-specific antibody small angle scattering data  s, nm-1
ln I(s)
Tri-specific antibody Guinier plot ln 1.21×10-1 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Tri-specific antibody Kratky plot 1.104 0 √3 sRg
p(r)
Tri-specific antibody pair distance distribution function Rg: 4.1 nm 0 Dmax: 13 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Tri-specific antibody OTHER model
log I(s)
 s, nm-1
Tri-specific antibody SREFLEX model
Synchrotron SAXS data were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using an Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09524 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were collected with an in-line size exclusion chromatography column (Shodex KW403, Shanghai, China) equilibrated in 20mM Sodium Phosphate, pH7.4, 150mM sodium chloride. 35μL of sample at a concentration of ~5 mg/ml was run at a flowrate of 0.16 ml/min and 3 s X-ray exposures were collected continuously during a 30min elution, resulting in 619 frames. Images were corrected, normalised and processed into 1D curves using GDA and DAWN at the Diamond Light Source.

Tri-specific antibody (UCB0159)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   102.3 kDa
Sequence   FASTA