Structural and functional properties of mouse proNGF.

Paoletti F, Konarev PV, Covaceuszach S, Schwarz E, Cattaneo A, Lamba D, Svergun DI, Biochem Soc Trans 34(Pt 4):605-6 (2006) Europe PMC

SASDM87 – Mouse ProNGF - pro-form of the NGF (nerve growth factor)

Mouse ProNGF - pro-form of the NGF (nerve growth factor)
MWI(0) 0 kDa
MWexpected 100 kDa
VPorod 97 nm3
log I(s) 9.78×101 9.78×100 9.78×10-1 9.78×10-2
Mouse ProNGF - pro-form of the NGF (nerve growth factor) small angle scattering data  s, nm-1
ln I(s)
Mouse ProNGF - pro-form of the NGF (nerve growth factor) Guinier plot ln 9.79×101 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Mouse ProNGF - pro-form of the NGF (nerve growth factor) Kratky plot 1.104 0 3 sRg
p(r)
Mouse ProNGF - pro-form of the NGF (nerve growth factor) pair distance distribution function Rg: 3.2 nm 0 Dmax: 11 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Mouse ProNGF - pro-form of the NGF (nerve growth factor) DAMMIN model

Synchrotron SAXS data from solutions of Mouse ProNGF - pro-form of the NGF (nerve growth factor) in 50 mM Na-phosphate, pH 7 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6.4 and 12.8 mg/ml were measured at 25°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Mouse ProNGF - pro-form of the NGF (nerve growth factor) (proNGF)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   49.9 kDa
Sequence   FASTA