The Relationship of Precursor Cluster Concentration in a Saturated Crystallization Solution to Long-Range Order During the Transition to the Solid Phase.

Marchenkova MA, Boikova AS, Ilina KB, Konarev PV, Pisarevsky YV, Dyakova YA, Kovalchuk MV, Acta Naturae 15(1):58-68 (2023) Europe PMC

SASDM89 – Lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2 (mixture of monomers, dimers and octamers, with octamer volume fractions from 0.9% to 4.4%)

Lysozyme C
MWexperimental 27 kDa
MWexpected 14 kDa
log I(s) 1.93×10-2 1.93×10-3 1.93×10-4 1.93×10-5
Lysozyme C small angle scattering data  s, nm-1
ln I(s)
Lysozyme C Guinier plot ln 1.94×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Lysozyme C Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Lysozyme C PDB (PROTEIN DATA BANK) model
Lysozyme C PDB (PROTEIN DATA BANK) model
Lysozyme C PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of lysozyme crystallization solutions with precipitants in 100 mM sodium acetate, pH 4.6, 2.0 M sodium formate, pH 4.6 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 20.00 mg/ml was measured at 20°C. 20 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental SAXS data set from lysozyme crystallization solutions with precipitants from crystallization kits CS 1 and CS2. The oligomeric composition (volume fractions of monomers, dimers, and octamers) were estimated by OLIGOMER. Volume fractions of octamers are within 0.9% and 4.4 %. An additional zip file made available in the full entry archive contains the OLIGOMER fit files, volume fractions and a Table key with information about the buffer composition.

Lysozyme C (lysozyme)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   14.3 kDa
 
UniProt   P00698 (19-129)
Sequence   FASTA
 
PDB ID   1LYS