| MWexperimental | 32 | kDa |
| MWexpected | 29 | kDa |
| VPorod | 39 | nm3 |
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log I(s)
6.73×105
6.73×104
6.73×103
6.73×102
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s, nm-1
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Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment.
Paoletti F, Malerba F, Konarev PV, Visintin M, Scardigli R, Fasulo L, Lamba D, Svergun DI, Cattaneo A, Arch Biochem Biophys 522(1):26-36 (2012) Europe PMCSASDM97 – Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)
Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)
in
phosphate buffered saline, pH 7
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.4 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.40 mg/ml was measured
at 25°C.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tags:
X33
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