The structure of MadC from Clostridium maddingley reveals new insights into class I lanthipeptide cyclases

Knospe C, Kamel M, Spitz O, Hoeppner A, Galle S, Reiners J, Kedrov A, Smits S, Schmitt L, Frontiers in Microbiology 13 (2023) DOI

SASDMT7 – Lanthionine synthetase C-like protein (MadC)

Lanthionine synthetase C-like protein
MWI(0) 46 kDa
MWexpected 50 kDa
VPorod 78 nm3
log I(s) 3.32×10-2 3.32×10-3 3.32×10-4 3.32×10-5
Lanthionine synthetase C-like protein small angle scattering data  s, nm-1
ln I(s)
Lanthionine synthetase C-like protein Guinier plot ln 3.33×10-2 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Lanthionine synthetase C-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Lanthionine synthetase C-like protein pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Lanthionine synthetase C-like protein GASBOR model
SAXS data from solutions of Lanthionine synthetase C-like protein (MadC) in 50 mM HEPES, 500 mM NaCl, pH 8 were collected using a Xenocs Xeuss 2.0 Q-Zoom instrument (Center for Structural Studies, Heinrich-Heine-University, Düsseldorf, Germany) equipped with a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 13.80 mg/ml was measured at 10°C. 12 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Lanthionine synthetase C-like protein (MadC)
Mol. type   Protein
Organism   Clostridium sp. Maddingley MBC34-26
Olig. state   Monomer
Mon. MW   49.7 kDa
 
UniProt   K6THC9 (1-438)
Sequence   FASTA