| MWexperimental | 140 | kDa |
| MWexpected | 91 | kDa |
| VPorod | 267 | nm3 |
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log I(s)
3.40×102
3.40×101
3.40×100
3.40×10-1
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s, nm-1
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Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering.
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI, J Biol Chem 284(37):25334-42 (2009) Europe PMCSASDN53 – Ternary Human Pex5p(full-length)-Pex14p(N)-PTS1 Protein Complex (1:7:1 stoichiometry)
Peroxisomal targeting signal 1 receptorPeroxisomal membrane protein PEX14
PTS1-BP
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Fits and models
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Synchrotron SAXS
data from solutions of
Ternary Human Pex5p(full-length)-Pex14p(N)-PTS1 Protein Complex (1:7:1 stoichiometry)
in
50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.1 and 11 mg/ml were measured
at 15°C.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
X33
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