data from solutions of
Acinetobacter baumannii putrescine N-hydroxylase, 1 mg/mL
25 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP, pH 7.5
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.1234 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.00 mg/ml was measured
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The deposited scattering curve was averaged using FrameSlice, using frames 1-2 in the Guinier region and frames 1-30 in the other q regions. The first 5 (lowest q) data points were omitted based on analysis with Primus AutoRg.