Divergent kinase WNG1 is regulated by phosphorylation of an atypical activation sub-domain.

Dewangan PS, Beraki TG, Paiz EA, Appiah Mensah D, Chen Z, Reese ML, Biochem J (2022) Europe PMC

SASDNT5 – Bradyzoite Pseudokinase 1 (amino acids 61-377)

Rhoptry kinase family protein
MWexperimental 148 kDa
MWexpected 143 kDa
VPorod 207 nm3
log I(s) 6.74×10-1 6.74×10-2 6.74×10-3 6.74×10-4
Rhoptry kinase family protein small angle scattering data  s, nm-1
ln I(s)
Rhoptry kinase family protein Guinier plot ln 6.74×10-1 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Rhoptry kinase family protein Kratky plot 1.104 0 √3 sRg
p(r)
Rhoptry kinase family protein pair distance distribution function Rg: 3.7 nm 0 Dmax: 11.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
log I(s)
 s, nm-1
Rhoptry kinase family protein DAMMIN model
log I(s)
 s, nm-1
Rhoptry kinase family protein SASREF model
Synchrotron SAXS data from solutions of Bradyzoite Pseudokinase 1 (amino acids 61-377) in 20 mM HEPES, 100 mM NaCl, pH 7.5 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a wavelength of λ = 0.09537 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.70 mg/ml was measured at 20°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

BPK1 is protein secreted by apicomplexan parasite Toxoplasma gondii from its dense granules, when it has infected the host cell. The BPK1 is a part of the cyst wall in the bradyzoite stage of Toxoplasma. It binds to other proteins and maintains the integrity of the bradyzoite cyst wall.

Rhoptry kinase family protein (BPK1)
Mol. type   Protein
Organism   Toxoplasma gondii (strain ATCC 50853 / GT1)
Olig. state   Tetramer
Mon. MW   35.8 kDa
 
UniProt   S7UMR4 (61-377)
Sequence   FASTA