BTB protein domains of the Tramtrack group are Arthropoda-specific multimerization modules

Artem Bonchuk.

SASDP49 – BTB domain of CG6765 protein at 1.5 mg/ml

Uncharacterized protein, isoform A
MWexperimental 103 kDa
MWexpected 92 kDa
VPorod 166 nm3
log I(s) 8.89×101 8.89×100 8.89×10-1 8.89×10-2
Uncharacterized protein, isoform A small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein, isoform A Guinier plot ln 8.89×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein, isoform A Kratky plot 1.104 0 3 sRg
Dmax: 12.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Uncharacterized protein, isoform A ALPHAFOLD model

Synchrotron SAXS data from solutions of the BTB domain of CG6765 protein in 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 10°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Uncharacterized protein, isoform A (CG6765)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Hexamer
Mon. MW   15.3 kDa
 
UniProt   Q9VSL1 (1-133)
Sequence   FASTA