The Arthropoda-specific Tramtrack group BTB protein domains use previously unknown interface to form hexamers.

Bonchuk AN, Balagurov KI, Baradaran R, Boyko KM, Sluchanko NN, Khrustaleva AM, Burtseva AD, Arkova OV, Khalisova KK, Popov VO, Naschberger A, Georgiev PG, Elife 13 (2024) Europe PMC

SASDP49 – BTB domain of CG6765 protein at 1.5 mg/ml

Uncharacterized protein, isoform A
MWexperimental 103 kDa
MWexpected 92 kDa
VPorod 166 nm3
log I(s) 8.89×101 8.89×100 8.89×10-1 8.89×10-2
Uncharacterized protein, isoform A small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein, isoform A Guinier plot ln 8.89×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein, isoform A Kratky plot 1.104 0 √3 sRg
Dmax: 12.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein, isoform A ALPHAFOLD model
Synchrotron SAXS data from solutions of the BTB domain of CG6765 protein in 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 10°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Uncharacterized protein, isoform A (CG6765)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Hexamer
Mon. MW   15.3 kDa
 
UniProt   Q9VSL1 (1-133)
Sequence   FASTA