Synchrotron SAXS data from solutions of glucose isomerase in 25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH 7 were collected on the ID7A1 BioSAXS / HP-Bio Beamline beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.089 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 17.9 mg/ml was injected onto a column at 23°C. 82 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.