Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D, Commun Biol 6(1):1057 (2023) Europe PMC

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

Spike glycoprotein (ACE2 receptor binding domain)
MWexperimental 44 kDa
MWexpected 29 kDa
log I(s) 3.11×103 3.11×102 3.11×101 3.11×100
Spike glycoprotein (ACE2 receptor binding domain) small angle scattering data  s, nm-1
ln I(s)
Spike glycoprotein (ACE2 receptor binding domain) Guinier plot ln 3.12×103 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Spike glycoprotein (ACE2 receptor binding domain) Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Scattering data from solutions of SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL in phosphate buffered saline, pH 7.4 were collected using a AGIPD-1Mpix detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RBD solutions were measured on the European XFEL using a "reservoir" to inject the sample into an in-vacuum liquid jet delivery system.

Spike glycoprotein (ACE2 receptor binding domain) (RBD SARS-CoV-2)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   29.3 kDa
 
UniProt   P0DTC2 (319-566)
Sequence   FASTA