Small Angle Scattering Biological Data Bank SASBDB


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105 hits found for Blanchet

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS

ATP-citrate synthase experimental SAS data

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K

R_g^Guinier	6.1	nm
D_max	19.0	nm
Volume^Porod	765	nm³

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

R_g^Guinier	6.2	nm
D_max	19.0	nm
Volume^Porod	787	nm³

SASDFC3 – Human ATP-citrate synthase (ACLY) full length in HBS + Citrate + Coenzyme-A

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

R_g^Guinier	5.9	nm
D_max	17.0	nm
Volume^Porod	775	nm³

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data

Sample:	Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
...Blanchet CE, Langley DB, Whitten AE, Svergun DI

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	100	nm³

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data

Sample:	Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer:	25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23

R_g^Guinier	3.9	nm
D_max	12.7	nm
Volume^Porod	202	nm³

SASDAF4 – DmMfe2

Peroxisomal multifunctional enzyme type 2 experimental SAS data

Peroxisomal multifunctional enzyme type 2 Kratky plot

Sample:	Peroxisomal multifunctional enzyme type 2 dimer, 128 kDa Drosophila melanogaster protein
Buffer:	20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 12

Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering. FEBS Lett 587(4):305-10 (2013)
...Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T

R_g^Guinier	3.6	nm
D_max	12.0	nm

SASDAG4 – HsMfe2

Sample:	Peroxisomal multifunctional enzyme type 2 dimer, 159 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 22

R_g^Guinier	4.6	nm
D_max	15.0	nm

SASDAJ4 – CRM1

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3

Structural determinants and mechanism of mammalian CRM1 allostery. Structure 21(8):1350-60 (2013)
...Blanchet CE, Voß B, Haselbach D, Kappel C, Monecke T, Svergun DI, Stark H, Ficner R, Zachariae U, Grubmüller H, Dickmanns A

R_g^Guinier	3.8	nm
D_max	11.0	nm
Volume^Porod	190	nm³

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data

Sample:	Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
...Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	92	nm³

SASDAK4 – CRM1 RanGTP

Exportin-1GTP-binding nuclear protein Ran experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3

R_g^Guinier	3.6	nm
D_max	10.0	nm

SASDAL4 – CRM1 RanGTP Snu1

Exportin-1GTP-binding nuclear protein RanSnurportin-1 experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein GTP-binding nuclear protein Ran monomer, 24 kDa Homo sapiens protein Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Feb 3

R_g^Guinier	4.1	nm
D_max	14.0	nm

SASDAM4 – CRM1 Snu1

Exportin-1Snurportin-1 experimental SAS data

Sample:	Exportin-1 monomer, 123 kDa Mus musculus protein Snurportin-1 monomer, 41 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 10

R_g^Guinier	4.3	nm
D_max	15.0	nm

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Hammel M, Hopkins J, Huang Q, Hub J, H...

R_g^Guinier	1.5	nm
D_max	4.9	nm
Volume^Porod	18	nm³

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	220	nm³

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.3	nm
D_max	10.1	nm
Volume^Porod	243	nm³

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.6	nm
D_max	5.1	nm
Volume^Porod	27	nm³

SASDPT4 – Consensus SAXS Profile - Lysozyme

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	19	nm³

SASDQT4 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P

R_g^Guinier	4.5	nm
D_max	20.0	nm
Volume^Porod	454	nm³

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.4	nm
D_max	4.4	nm

SASDQU4 – DNA-binding protein from starved cells: DgrDpsWT + 6 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	18.4	nm
Volume^Porod	440	nm³

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.2	nm
D_max	3.8	nm

SASDQV4 – DNA-binding protein from starved cells: DgrDpsWT + 12 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	15.5	nm
Volume^Porod	464	nm³

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.4	nm

SASDQW4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.1	nm
Volume^Porod	439	nm³

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.3	nm

SASDQX4 – DNA-binding protein from starved cells: DgrDpsWT + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.4	nm
Volume^Porod	427	nm³

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.5	nm

SASDQY4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl, 5 mM EDTA

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS, 230 mM NaCl, 5 mM EDTA, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	453	nm³

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.4	nm
D_max	4.8	nm

SASDQZ4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	13.9	nm
Volume^Porod	458	nm³

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.5	nm
D_max	4.1	nm

SASDQ25 – DNA-binding protein from starved cells: DgrDpsWT + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.3	nm
Volume^Porod	438	nm³

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.6	nm
D_max	4.3	nm

SASDQ35 – DNA-binding protein from starved cells: DgrDps-D43A mutant in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.8	nm
Volume^Porod	451	nm³

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.2	nm
D_max	9.1	nm

SASDQ45 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.1	nm
Volume^Porod	412	nm³

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.3	nm
D_max	9.7	nm

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	20.7	nm
Volume^Porod	409	nm³

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	404	nm³

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	431	nm³

SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot

Sample:	Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 8

Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H

R_g^Guinier	2.1	nm
D_max	6.8	nm
Volume^Porod	50	nm³

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data

Photinus pyralis firefly luciferase Kratky plot

Sample:	Photinus pyralis firefly luciferase , 61 kDa Photinus pyralis protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
...Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease , 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 6

R_g^Guinier	6.0	nm
D_max	17.5	nm
Volume^Porod	738	nm³

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 6

R_g^Guinier	6.1	nm
D_max	17.5	nm
Volume^Porod	747	nm³

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV)

mock recombinant baculovirus experimental SAS data

mock recombinant baculovirus Kratky plot

Sample:	mock recombinant baculovirus , 0 kDa unidentified baculovirus
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE56 – Human ATP-citrate synthase (ACLY) in HBS + Citrate + Coenzyme-A

Sample:	ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 5

R_g^Guinier	5.8	nm
D_max	16.5	nm
Volume^Porod	709	nm³

SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells experimental SAS data

Sample:	High Five insect cells , 0 kDa Trichoplusia ni
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE66 – C. limicola ATP-citrate lyase (ACL) in HBS

Sample:	ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer:	20mM HEPES, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

R_g^Guinier	6.1	nm
D_max	20.0	nm
Volume^Porod	666	nm³

SASDH66 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE76 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate

Sample:	ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

R_g^Guinier	6.0	nm
D_max	20.0	nm
Volume^Porod	672	nm³

SASDH76 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Sample:	Cathepsin B-like cysteine protease , 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDE86 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A

Sample:	ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer:	20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4

R_g^Guinier	5.7	nm
D_max	17.5	nm
Volume^Porod	791	nm³

SASDF86 – Human Galectin-10 (Tyr69Glu mutant)

Galectin-10 Tyr69Glu experimental SAS data

Sample:	Galectin-10 Tyr69Glu dimer, 33 kDa Homo sapiens protein
Buffer:	20 mM Hepes 150 NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Feb 4

Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science 364(6442) (2019)
...Blanchetot C, Saunders M, Hammad H, Savvides SN, Lambrecht BN

R_g^Guinier	2.1	nm
D_max	8.2	nm
Volume^Porod	46	nm³

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Sample:	Cathepsin B-like cysteine protease , 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Cathepsin B-like cysteine protease , 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDA97 – PlaB

Sample:	PlaB tetramer, 220 kDa Legionella pneumophila protein
Buffer:	100 mM Tris 100 mM Nacl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 15

Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. Sci Rep 5:10734 (2015)
...Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI

R_g^Guinier	4.0	nm
D_max	10.5	nm
Volume^Porod	270	nm³

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	1.5	nm
D_max	4.6	nm
Volume^Porod	16	nm³

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	16	nm³

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data

Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot

Sample:	Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.2	nm
D_max	8.8	nm
Volume^Porod	221	nm³

SASDSH7 – Cationic liposomes containing DOTMA:DOPE (2:1) with negatively charged messenger RNA (mRNA) (raito 0.65:1)

mRNA -- proprietary sequence(R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride1,2-dioleoyl-sn-glycero-3-phosphoethanolamine experimental SAS data

Sample:	mRNA -- proprietary sequence , 400 kDa RNA (R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride , 1 kDa lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine , 1 kDa lipid
Buffer:	10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jun 9

Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
...Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.2	nm
D_max	9.0	nm
Volume^Porod	214	nm³

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.2	nm
D_max	14.1	nm
Volume^Porod	228	nm³

SASDPL7 – Xylose isomerase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.3	nm
D_max	14.1	nm
Volume^Porod	233	nm³

SASDQL7 – Bicelles formed by DMPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot

Sample:	DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 1

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	1.6	nm
D_max	4.8	nm
Volume^Porod	25	nm³

SASDQM7 – Bicelles formed by DMPC cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid experimental SAS data

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid Kratky plot

Sample:	DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	1.6	nm
D_max	4.5	nm
Volume^Porod	24	nm³

SASDQN7 – Bicelles formed by DMPC CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate experimental SAS data

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate Kratky plot

Sample:	DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQP7 – Bicelles formed by DMPC DMPG and CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data

Sample:	DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQQ7 – Bicelles formed by DMPC DMPG and cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data

Sample:	DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQR7 – Bicelles formed by DMPC DMPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data

Sample:	DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQS7 – Bicelles formed by POPC CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot

Sample:	CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQT7 – Bicelles formed by POPC cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot

Sample:	Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQU7 – Bicelles formed by POPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot

Sample:	DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQV7 – Bicelles formed by POPC POPG and CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data

Sample:	CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQW7 – Bicelles formed by POPC POPG and cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data

Sample:	Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDQX7 – Bicelles formed by POPC POPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data

Sample:	DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer:	Tris buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
...Blanchet C, Roosen-Runge F, Cárdenas M

SASDNY7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 50 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A

R_g^Guinier	4.4	nm
D_max	16.1	nm
Volume^Porod	409	nm³

SASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 80 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	17.1	nm
Volume^Porod	475	nm³

SASDN28 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	438	nm³

SASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 480 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.8	nm
D_max	20.5	nm
Volume^Porod	430	nm³

SASDN48 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 50 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	9.3	nm
Volume^Porod	290	nm³

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	8.8	nm
Volume^Porod	291	nm³

SASDCQ8 – Bovine serum albumin (BSA)

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	TRIS 50mM, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21

A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet

R_g^Guinier

2.8

SASDRQ8 – Bovine serum albumin monomer separated using asymmetric flow field flow fractionation (AFFFF)

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	104	nm³

SASDCR8 – Glucose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21

A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet

R_g^Guinier

2.7

SASDCS8 – Cytochrome C

Sample:	Cytochrome C monomer, 12 kDa Bos taurus protein
Buffer:	TRIS 50mM, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21

A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet

R_g^Guinier

1.3

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...

R_g^Guinier

3.1

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

5.8

SASDQ29 – Thyroglobulin - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Thyroglobulin monomer, 303 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

7.0

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Spike glycoprotein (ACE2 receptor binding domain) Kratky plot

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

3.0

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Blanchet C, Svergun D, Jeffries C

R_g^Guinier	1.8	nm
D_max	5.1	nm
Volume^Porod	37	nm³

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