SAXS data from solutions of the NPL3 protein RNA recognition motif domains 1 and 2 (RRM1,2) in 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH 6.5 were collected using a Rigaku bioSAXS-1000 instrument at the Technische Universität München (TUM; Garching, Germany) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.90 mg/ml was measured at 25°C. Eight successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample was dialyzed in same buffer before the measurements.