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3 hits found for Serine/arginine (SR)-type shuttling mRNA binding protein NPL3

SASDQT5NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 2.0 nm
Dmax 6.8 nm
VolumePorod 24 nm3

SASDQU5NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) P196D/A197D linker mutant

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 1.9 nm
Dmax 6.7 nm
VolumePorod 25 nm3

SASDQV5NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type, bound to RNA

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3Short RNA oligonucleotide (NPL3 binding sequence) experimental SAS data
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Short RNA oligonucleotide (NPL3 binding sequence) Kratky plot
Sample: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
...NPL3 binding sequence) monomer, 4 kDa RNA
Buffer: 20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 30 nm3