Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D, Commun Biol 6(1):1057 (2023) Europe PMC

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Albumin
MWexperimental 66 kDa
MWexpected 66 kDa
log I(s) 2.47×10-3 2.47×10-4 2.47×10-5 2.47×10-6
Albumin small angle scattering data  s, nm-1
ln I(s)
Albumin Guinier plot ln 2.47×10-3 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Albumin Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Scattering data from solutions of Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL in phosphate buffered saline, pH 7.4 were collected using a AGIPD-1Mpix detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.13 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

BSA samples were stored and directly injected into a continuous-flowing liquid jet XFEL sample delivery system using a HPLC auto sampler.

Albumin (BSA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   66.4 kDa
 
UniProt   P02769 (25-607)
Sequence   FASTA