The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements.

Korn SM, Dhamotharan K, Jeffries CM, Schlundt A, Nat Commun 14(1):3331 (2023) Europe PMC

SASDR63 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in HEPES conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2
MWexperimental 21 kDa
MWexpected 22 kDa
VPorod 35 nm3
log I(s) 6.84×10-2 6.84×10-3 6.84×10-4 6.84×10-5
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 small angle scattering data  s, nm-1
ln I(s)
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Guinier plot ln 6.85×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot 1.104 0 3 sRg
p(r)
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 pair distance distribution function Rg: 3.2 nm 0 Dmax: 11.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH 7.2 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 6.6 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 25°C. 2100 successive 0.99 second frames were collected through the SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. 45 sample frames from the SEC peak were selected for zero-concentration extrapolation using CHROMIXS in combination with PRIMUS.

The quoted experimental molecular weight was determined using MALLS/RI measurements. The data displayed in this entry were corrected for repulsive interparticle interferences via extrapolation to zero concentration. Additional SEC-SAXS (unsubtracted frames) and MALLS/RI data are made available in the full entry zip archive.

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 (SL4ext)
Mol. type   RNA
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Monomer
Mon. MW   22.1 kDa
Sequence   FASTA