Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation

Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M Vadas O, Gervasio F, Pellegrini E, Bowler M, Science 381(6663):1217-1225 (2023) DOI

SASDRC6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with ADP-AlF4

Mitogen-activated protein kinase 14
Dual specificity mitogen-activated protein kinase kinase 6
Adenosine 5'-Diphosphate Tetrafluoroaluminate
MWexperimental 65 kDa
MWexpected 83 kDa
VPorod 113 nm3
log I(s) 2.55×10-1 2.55×10-2 2.55×10-3 2.55×10-4
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate small angle scattering data  s, nm-1
ln I(s)
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate Guinier plot ln 2.56×10-1 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate Kratky plot 1.104 0 √3 sRg
p(r)
Mitogen-activated protein kinase 14 Dual specificity mitogen-activated protein kinase kinase 6 Adenosine 5'-Diphosphate Tetrafluoroaluminate pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.1 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions the MKK6GRA-p38α complex with ADP-AlF4 in 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ADP-AlF4 = Adenosine 5'-Diphosphate Tetrafluoroaluminate

Mitogen-activated protein kinase 14 (p38α, WT)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.5 kDa
 
UniProt   Q16539 (10-360)
Sequence   FASTA
 
Dual specificity mitogen-activated protein kinase kinase 6 (MKK6DDGRA)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   41.8 kDa
Sequence   FASTA
 
Adenosine 5'-Diphosphate Tetrafluoroaluminate (ADP ALF4)
Mol. type   Other
Olig. state   Monomer
Mon. MW   0.5 kDa
Chemical formula