Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles

Ehrit J, Gräwert T, Göddeke H, Konarev P Svergun D, Nagel N, Biophysical Journal (2023) DOI

SASDRP9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)
MWexperimental 42 kDa
MWexpected 45 kDa
log I(s) 4.75×10-3 4.75×10-4 4.75×10-5 4.75×10-6
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) small angle scattering data  s, nm-1
ln I(s)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Guinier plot ln 4.76×10-3 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) Kratky plot 1.104 0 √3 sRg
p(r)
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) pair distance distribution function Rg: 3.5 nm 0 Dmax: 8.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER [STATIC IMAGE] model
log I(s)
 s, nm-1
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) OTHER model
Synchrotron SAXS data from solutions of Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) in aqueous solution of 4% methanol, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tetradecanoic acid (myristic acid): https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:28875 Polysorbate 20: https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:53424

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) (PS20_HMA)
Mol. type   Other
Olig. state   0
Mon. MW   1.2 kDa
Chemical formula