| MWexperimental | 105 | kDa |
| MWexpected | 104 | kDa |
| VPorod | 176 | nm3 |
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log I(s)
5.41×101
5.41×100
5.41×10-1
5.41×10-2
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s, nm-1
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1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) as target for anti Toxoplasma gondii agents: crystal structure, biochemical characterisation and biological evaluation of inhibitors
Mazzone F, Hoeppner A, Reiners J Gertzen C, Applegate V, Abdullaziz M, Gottstein J, Degrandi D, Wesemann M, Kurz T, Smits S, Pfeffer K, Biochemical Journal (2024) DOISASDS47 – 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR)
1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of 1-Deoxy-D-xylulose-5-phosphate reductoisomerase in 20 mM Tris/HCl, 150 mM NaCl, 40 mM MgCl2, 2% glycerol,, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 10°C. 1500 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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