Intramolecular Structural Heterogeneity altered by Long-range Contacts in an Intrinsically Disordered Protein

Sagi Meir.

SASDS93 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain
MWexperimental 2 kDa
MWexpected 2 kDa
log I(s) 1.46×10-3 1.46×10-4 1.46×10-5 1.46×10-6
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain small angle scattering data  s, nm-1
ln I(s)
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Guinier plot ln 1.46×10-3 Rg: 1.2 nm 0 (1.2 nm)-2 s2
(sRg)2I(s)/I(0)
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram Rg, nm
Synchrotron SAXS data from solutions of Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain in 20 mM Tris, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 24°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN. Number of frames = UNKNOWN

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain (S(26-45))
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   2.1 kDa
Sequence   FASTA