Intramolecular Structural Heterogeneity altered by Long-range Contacts in an Intrinsically Disordered Protein

Sagi Meir

Submitted to SASBDB: 2023 May 22 Published in SASBDB: 2023 May 22

SASDS93 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSA3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSB3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSC3 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSD3 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSE3 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.0 nm

SASDSF3 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSG3 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSH3 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSJ3 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSK3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSL3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSM3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSN3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSP3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSQ3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDSR3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSS3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDST3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSU3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDSV3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSW3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDSX3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDSY3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.3 nm

SASDSZ3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDS24 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Sep 15 RgGuinier 1.1 nm

SASDS34 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDS44 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDS54 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDS64 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDS74 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDS84 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDS94 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Sep 15 RgGuinier 1.3 nm

SASDSA4 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSB4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSC4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSD4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSE4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSF4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSG4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSH4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSJ4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSK4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSL4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.2 nm

SASDSM4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSN4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSP4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 1 M NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15 RgGuinier 1.1 nm

SASDSQ4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm

SASDSR4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSS4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDST4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSU4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.3 nm

SASDSV4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.0 nm

SASDSW4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSX4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 500 mM NaCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.1 nm

SASDSY4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Apr 20 RgGuinier 1.2 nm