Intramolecular Structural Heterogeneity altered by Long-range Contacts in an Intrinsically Disordered Protein

Sagi Meir.

SASDSH3 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain
MWexperimental 2 kDa
MWexpected 2 kDa
log I(s) 1.20×103 1.20×102 1.20×101 1.20×100
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain small angle scattering data  s, nm-1
ln I(s)
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Guinier plot ln 1.20×103 Rg: 1.2 nm 0 (1.2 nm)-2 s2
(sRg)2I(s)/I(0)
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram Rg, nm
Synchrotron SAXS data from solutions of Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain in 20 mM Tris, pH 8 were collected on the EMBL P12 beam line at the PETRA III, DESY storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain (S(129-146))
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   2.4 kDa
Sequence   FASTA