Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein.

Koren G, Meir S, Holschuh L, Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R, Proc Natl Acad Sci U S A 120(30):e2220180120 (2023) Europe PMC

SASDSQ4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain
MWexperimental 2 kDa
MWexpected 2 kDa
log I(s) 7.68×102 7.68×101 7.68×100 7.68×10-1
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain small angle scattering data  s, nm-1
ln I(s)
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Guinier plot ln 7.69×102 Rg: 1.2 nm 0 (1.2 nm)-2 s2
(sRg)2I(s)/I(0)
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram Rg, nm
Synchrotron SAXS data from solutions of Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain in 20 mM Tris, 3 M GdnHCl, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain (S(110-125))
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   2.0 kDa
Sequence   FASTA