SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

DUF507 family protein

MW^experimental	21	kDa
MW^expected	22	kDa
V^Porod	39	nm³

log I(s) 4.62×10⁰ 4.62×10^-1 4.62×10^-2 4.62×10^-3

DUF507 family protein small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 4.63×10⁰ R_g: 2.3 nm 0 (2.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 2.4 nm 0 D_max: 9 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.4

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.275
0.074

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

DUF507 family protein CUSTOM IN-HOUSE model

Synchrotron SAXS data from solutions of Aquifex aeolicus domain of unknown function 507 in 20 mM Hepes pH 7.2, 400 mM NaCl, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1234 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DUF507 family protein (AaDUF507)
Mol. type		Protein
Organism		Aquifex aeolicus (strain VF5)
Olig. state		Monomer
Mon. MW		21.9 kDa

UniProt		O67633 (1-183)
Sequence		FASTA