Specific phosphoinositide interaction of Jps1 is a key feature during unconventional secretion in Ustilago maydis

Dali S, Schultz M, Köster M, Kamel M, Busch M, Steinchen W, Hänsch S, Papadopoulos A, Reiners J, Smits S, Kedrov A, Altegoer F, Schipper K, Journal of Biological Chemistry :110215 (2025) DOI

SASDT97 – Ustilago maydis Jps1 protein

Uncharacterized protein
MWexperimental 120 kDa
MWexpected 122 kDa
VPorod 254 nm3
log I(s) 9.30×10-2 9.30×10-3 9.30×10-4 9.30×10-5
Uncharacterized protein small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein Guinier plot ln 9.30×10-2 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized protein pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein CORAL model
Synchrotron SAXS data from solutions of Jps1 protein in 20 mM HEPES, 20 mM KCl, 200 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

AlphaFold Protein Structure Database entry, full-length monomer: https://alphafold.ebi.ac.uk/entry/A0A0D1C3B2

Uncharacterized protein (Jps1 (1-484))
Mol. type   Protein
Organism   Ustilago maydis (strain 521 / FGSC 9021)
Olig. state   Dimer
Mon. MW   60.8 kDa
 
UniProt   A0A0D1C3B2 (1-484)
Sequence   FASTA