The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry

Kocher F, Applegate V, Reiners J Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K, Nature Communications 15(1) (2024) DOI

SASDTQ5 – SemDΔAPH (apo form)

Uncharacterized protein
MWexperimental 37 kDa
MWexpected 35 kDa
VPorod 67 nm3
log I(s) 3.22×103 3.22×102 3.22×101 3.22×100
Uncharacterized protein small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein Guinier plot ln 3.22×103 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized protein pair distance distribution function Rg: 3.0 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein CORAL model
Synchrotron SAXS data from solutions of SemDΔAPH in 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3% glycerol, pH 8.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 8 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2400 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

AlphaFold Protein Structure Database, monomer: https://www.uniprot.org/uniprotkb/Q9Z7M7/entry

Uncharacterized protein (SemDΔAPH)
Mol. type   Protein
Organism   Chlamydia pneumoniae
Olig. state   Monomer
Mon. MW   35.1 kDa
 
UniProt   Q9Z7M7 (67-382)
Sequence   FASTA