Sugar binding to Kluyveromyces lactis glucokinase 1 (KlGlk1)

Renato H. Weiße.

SASDU36 – Kluyveromyces lactis glucokinase 1 (KlGlk1), 1.0 mg/mL, no ligand

Glucokinase-1
Glucokinase-1
MWexperimental 74 kDa
MWexpected 161 kDa
VPorod 128 nm3
log I(s) 4.72×10-2 4.72×10-3 4.72×10-4 4.72×10-5
Glucokinase-1 Glucokinase-1 small angle scattering data  s, nm-1
ln I(s)
Glucokinase-1 Glucokinase-1 Guinier plot ln 4.72×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Glucokinase-1 Glucokinase-1 Kratky plot 1.104 0 √3 sRg
Dmax: 9.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Glucokinase-1 Glucokinase-1 PYMOL model
Glucokinase-1 Glucokinase-1 PYMOL model
Glucokinase-1 Glucokinase-1 PYMOL model
Glucokinase-1 Glucokinase-1 PYMOL model
Synchrotron SAXS data from solutions of Kluyveromyces lactis glucokinase 1 (KlGlk1), 1.0 mg/mL, no ligand in 10 mM Tris-HCl, 1 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.12399 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 30 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Glucokinase-1 (GLK1)
Mol. type   Protein
Organism   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Olig. state   Monomer
Mon. MW   53.8 kDa
 
UniProt   Q6CUZ3 (1-481)
Sequence   FASTA
 
Glucokinase-1 (GLK1)
Mol. type   Protein
Organism   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Olig. state   Dimer
Mon. MW   53.8 kDa
 
UniProt   Q6CUZ3 (1-481)
Sequence   FASTA