Alu RNA pseudoknot alterations influence SRP9/SRP14 association.

Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA, RNA (2025) Europe PMC

SASDU59 – BC120 RNA

BC120 RNA monomer
MWexperimental 43 kDa
MWexpected 39 kDa
VPorod 57 nm3
log I(s) 1.23×10-1 1.23×10-2 1.23×10-3 1.23×10-4
BC120 RNA monomer small angle scattering data  s, nm-1
ln I(s)
BC120 RNA monomer Guinier plot ln 1.24×10-1 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
BC120 RNA monomer Kratky plot 1.104 0 √3 sRg
p(r)
BC120 RNA monomer pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
BC120 RNA monomer DAMMIN model
log I(s)
 s, nm-1
BC120 RNA monomer DAMMIN model
log I(s)
 s, nm-1
BC120 RNA monomer DAMMIN model
Synchrotron SAXS data from solutions of BC120 RNA in phosphate buffered saline, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

BC120 RNA monomer
Mol. type   RNA
Olig. state   Monomer
Mon. MW   38.9 kDa
Sequence   FASTA