Alu RNA pseudoknot alterations influence SRP9/SRP14 association.

Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA, RNA (2025) Europe PMC

SASDU69 – EB120 RNA

EB120 RNA monomer
MWexperimental 56 kDa
MWexpected 39 kDa
VPorod 79 nm3
log I(s) 2.96×10-2 2.96×10-3 2.96×10-4 2.96×10-5
EB120 RNA monomer small angle scattering data  s, nm-1
ln I(s)
EB120 RNA monomer Guinier plot ln 2.96×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
EB120 RNA monomer Kratky plot 1.104 0 √3 sRg
p(r)
EB120 RNA monomer pair distance distribution function Rg: 4.3 nm 0 Dmax: 13.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
EB120 RNA monomer DAMMIN model
log I(s)
 s, nm-1
EB120 RNA monomer DAMMIN model
log I(s)
 s, nm-1
EB120 RNA monomer DAMMIN model
Synchrotron SAXS data from solutions of EB120 RNA in phosphate buffered saline, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.094 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

EB120 RNA monomer (EB120)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   38.8 kDa
Sequence   FASTA