|
Synchrotron SAXS
data from solutions of
Kluyveromyces lactis glucokinase 1 mutant H304Q, 7.0 mg/mL, 10 mM fructose
in
10 mM Tris-HCl, 1 mM DTT, 10 mM D-fructose, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.1239 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 14.2 and 7 mg/ml were measured
at 10°C.
30 successive
0.045 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION: Severe repulsive interparticle interference effects present in the sample result in inaccurate structural parameters (Rg, I(0), MW, Dmax) extracted from the data.
|
|
Glucokinase-1 (H304Q)
(KlGlk1-H304Q)
|
| Mol. type |
|
Protein |
| Organism |
|
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) |
| Olig. state |
|
Monomer |
| Mon. MW |
|
53.7 kDa |
| |
| UniProt |
|
Q6CUZ3
(1-481)
|
| Sequence |
|
FASTA |
| |
|
Glucokinase-1 (H304Q)
(KlGlk1-H304Q)
|
| Mol. type |
|
Protein |
| Organism |
|
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) |
| Olig. state |
|
Dimer |
| Mon. MW |
|
53.7 kDa |
| |
| UniProt |
|
Q6CUZ3
(1-481)
|
| Sequence |
|
FASTA |
| |
|
s, nm-1
