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Synchrotron SAXS
data from solutions of
Monomeric Kluyveromyces lactis glucokinase 1 triple point mutant (I356D, Y419D, H420D), 2.5 mg/mL, 10 mM glucose
in
10 mM Tris/HCl, 1 mM DTT, 10 mM D-glucose, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.12398 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.5 and 2.5 mg/ml were measured
at 10°C.
30 successive
0.045 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Data set merged from three independent measurements of the same sample. CAUTION: Repulsive interparticle interference effects present in the sample result in inaccurate structural parameters (Rg, I(0), MW, Dmax) extracted from the data.
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s, nm-1
