| MWexperimental | 52 | kDa |
| MWexpected | 51 | kDa |
| VPorod | 161 | nm3 |
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log I(s)
3.74×103
3.74×102
3.74×101
3.74×100
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s, nm-1
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Preorganized cyclic modules facilitate the self-assembly of protein nanostructures
Snoj J, Lapenta F, Jerala R, Chemical Science (2024) DOISASDUQ2 – DiTET-nn (coiled coil protein origami assembled from two linear chains)
RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)
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Fits and models
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Synchrotron SAXS data from solutions of DiTET-nn in 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 80.00 μl sample at 16.4 mg/ml was injected at a 0.75 ml/min flow rate onto a Cytiva Superdex 200 10/300 column at 20°C. 2400 successive 0.995 second frames were collected through the entire SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The experimental molecular weight quoted in this entry was evaluated using SEC-MALS combined with the refractive index (RI) measured before the SAXS measurements. |
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