| MWI(0) | 32 | kDa |
| MWexpected | 36 | kDa |
| VPorod | 58 | nm3 |
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log I(s)
3.14×10-2
3.14×10-3
3.14×10-4
3.14×10-5
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s, nm-1
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Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2.
Jamwal A, Colomb F, McSorley HJ, Higgins MK, Nat Commun 15(1):5226 (2024) Europe PMCSASDUZ6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3 bound to Interleukin-33
Interleukin-33 (L179V)Alarmin release inhibitor (Δ1-125; N175Q, N190Q)
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Fits and models
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Synchrotron SAXS data from solutions of the alarmin release inhibitor domains CCP2 and CCP3 bound to Interleukin-33 in 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH 7.2 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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