Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2.

Jamwal A, Colomb F, McSorley HJ, Higgins MK
Nat Commun 15(1):5226 (2024 Jun 19)

PMID: 38890291 doi: 10.1038/s41467-024-49550-0

Submitted to SASBDB: 2024 Apr 10 Published in SASBDB: 2024 Apr 25

SASDUW6 – Alarmin release inhibitor (HpARI2), full-length Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9 RgGuinier 2.6 nm Dmax 9.9 nm VolumePorod 31 nm3

SASDUX6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3 Sample: Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9 RgGuinier 2.3 nm Dmax 8.2 nm VolumePorod 28 nm3

SASDUY6 – Alarmin release inhibitor (HpARI2), full length, bound to Interleukin-33 Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9 RgGuinier 2.7 nm Dmax 9.7 nm VolumePorod 62 nm3

SASDUZ6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3 bound to Interleukin-33 Sample: Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9 RgGuinier 2.5 nm Dmax 8.3 nm VolumePorod 58 nm3